Research Pediatric Endocrinology Fellowship Top The Nemours division of endocrinology has a strong background in research, and has a wide variety of projects active at any given time. The training program has a special focus on clinical research training, with fellows functioning as integral members of the research team. Under the guidance of a faculty mentor, fellows will design and run their own projects, presenting their findings at national or international meetings and prepare a manuscript for publication. In their first year of training, fellows spend 20 percent of their time on research with 50 percent in their second year and 80 percent in their third year. With the available resources and ongoing projects, fellows are exposed to a wide range of endocrine research topics and have the opportunity to train in a wide range of techniques. Research Resources Clinical Research Center Wolfson Children's Hospital has a two-bed dedicated clinical research center with a designated area for a metabolic kitchen. Here, the research dietitian and dietary technician use the appropriate software for the estimating of nutrient contents, as well as for the preparation of menus. The CRC has breath collection equipment, infusion pumps, two indirect calorimeters, a glucose analyzer and a plasma/serum separation area. The unit also has EKG, pulse oximetry and automated vital signs equipment and a bicycle ergometer. A biodex dynamometer is located in the physical therapy department at the hospital, in close geographic proximity to the CRC. A Ph.D. level physical therapist is a consultant for studies involving muscle function. Percutaneous biopsy equipment is available to perform fat and muscle biopsies at the bedside. Three specially trained nurses with extensive experience staff the CRC. This resource allows for performance of complex endocrine and metabolic studies. Bone Mineral Density Lab A DXA scanner (Horizon A, Hologic) is owned by the Nemours research programs. It is located at Wolfson Children's Hospital and is staffed by technologists with extensive experience in bone densitometry and body composition. This lab provides fellows the unique exposure to reading and interpreting DXAs for research and clinical applications, a skill often tied to diagnostic radiology, but commonly run by endocrinologists. Biochemical Analysis Laboratory The Biomedical Analysis Lab at Nemours Children's Specialty Care occupies approximately 2,200 sq. ft. in the clinic building. The following equipment is housed in our laboratory: AB Sciex QTRAP 5500 LC/MS/MS with SelexION, two HP 5970 capillary GC/MS, a HP5973 capillary GC/MS with chemical ionization detector, a VG Optima Isochrome Model SIRA 3 magnetic sector IR/MS with GC/combustion and gas inlets, an Europa (Cheshire, England) GC/IRMS instrument a 5110A GC, a Beckman Model 6300 amino acid analyzer, immununoaffinity equipment and electrophoresis equipment. The lab is run by a Ph.D. biochemist with three technicians. With these resources, sensitive non-isotopic tracer metabolic studies can be performed. Cellular and Molecular Medicine Laboratory The ninth floor of Nemours Children's Specialty Care is devoted to laboratory space. Three full-time Ph.D. level researchers are available. There is a fully equipped cell culture facility, fluorescent microscopy and a complete molecular biology lab including facilities for PCR with real time fluorescent monitoring. In addition, we have access to flow cytometry through Wolfson Children's Hospital clinical lab. DNA sequencing is available through the University of Florida (Gainesville) and we conduct DNA microarray experiments in collaboration with Nemours Biomolecular Core Laboratory in Delaware. Computer facilities are available that are adequate to handle these enormous data sets. Through this lab, a wide variety of molecular and genetic studies can be done.